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Capacity of γ phage to form plaques on B. anthracis strains Sterne (pXO1+ pXO2−) and Ames (pXO1+ pXO2+). Tenfold dilutions of γ phage preparations (1010 PFU/ml; 100 μl) were spread on NBY agar with supplements that had been seeded with B. anthracis Sterne or Ames. Phage titers represent the number of phage particles applied to each plate. Capsule expression by the Ames strain prevented phage infection and plaque formation at all dilutions over the PFU range 109 to 105.
