Fig 4.

Phage-mediated hydrolysis of poly-γ-d-glutamic acid (PDGA). (A and B) Aliquots (1 × 10⁹ PFU) of γ phage (A) or ΦNIT1 phage (B) were incubated with 8 μg PDGA for 16 h at 37°C. Degradation of capsular material was determined by SDS-PAGE. Lanes: MW, molecular weight markers (in thousands); +ve, positive control (contains 35 μg CapD hydrolase [24] with PDGA); -ve, negative control (contains PDGA and no phage). PDGA was obtained from high-Mn-grown cultures of Bacillus licheniformis.