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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Arthritis Rheum. 2012 Aug;64(8):2734–2745. doi: 10.1002/art.34424

Figure 3. Wnt3a stimulates profibrotic gene expression and myofibroblast differentiation.

Figure 3

Confluent foreskin fibroblasts were infected with Ad-GFP or Ad-Wnt3a (30 MOI or as indicated) for up to 6 d. A. RNA was isolated at indicated intervals and subjected to real-time qPCR. Results, normalized with Gapdh, are shown as means ± SD of triplicates from three independent experiments. * p < 0.05. B. Whole cell lysates were subjected to Western blot analysis. Representative immunoblots. C. Fibroblasts were stained with DAPI (blue) or immunostained with antibodies against α-SMA (green). Original magnification × 400.