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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Arthritis Rheum. 2012 Aug;64(8):2734–2745. doi: 10.1002/art.34424

Figure 5. Wnt3a suppresses preadipocyte differentiation.

Figure 5

A. Human subcutaneous preadipocytes were incubated in PM-1 or DM-2 medium for 2 d, and infected with Ad-GFP or Ad-Wnt3a (30 MOI). Following a further 5 d, cells were fixed and stained with Oil Red O. Left panels, cytochemistry (original magnification 400×). Arrows indicate weak red staining in differentiated adipocytes. Right panel, quantitation of Oil Red O staining. 120 cells for each condition in five different fields (magnification 400×) were scored (N, none; +, small droplets, weak staining; ++, moderate staining; +++, large droplets). B. Preadipocytes were incubated in media with or without Wnt3a (100 ng/ml) for 7 days. RNA was isolated and subjected to real-time qPCR. The results, normalized with Gapdh, represent the means ± SD of triplicate determinations. * p<0.05. C. Preadipocytes were induced to adipogenic differentiation for 7 d. Cultures were then pretreated with SB431542 for 15 min and incubated with recombinant Wnt3a (100 ng/ml) for 24 h. RNA was isolated and subjected to real-time qPCR analysis. The results, normalized with Gapdh, represent the means ± SD of triplicate determinations. * p<0.05.