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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Arthritis Rheum. 2012 Aug;64(8):2734–2745. doi: 10.1002/art.34424

Figure 6. Wnt3a stimulates preadipocytes to myofibroblast differentiation.

Figure 6

Human subcutaneous preadipocytes were incubated in PM-1 or DM-2 medium for 4 d, and infected with Ad-GFP or Ad-Wnt3a (30 MOI) for 3 d. A. Whole cell lysates and supernatants were subjected to Western analysis. Representative Western blots. B. Cells were stained with DAPI (blue) or immunostained antibody against α-SMA (red). Original magnification × 400. C. Preadipocytes incubated in DM-2 medium for 7 d were incubated with recombinant Wnt3a (100 ng/ml) for 24 h in the presence or absence of SB431542. RNA was isolated and subjected to real-time qPCR analysis. The results, normalized with Gapdh, represent the means ± SD of triplicate determinations. * p<0.05. D. Mesenchymal progenitor cells (MPC) undergo PPARγ – dependent adipogenesis or TGF-β/Smad-dependent fibrogenesis (left panel). In the presence of Wnt3a (right panel), PPARγ is suppressed, while TGF-β is enhanced, resulting in repression of adipogenesis, myofibroblast transdifferentiation and enhanced fibrogenesis.