Table 3.
Main elution peaks obtained by reverse-phase HPLC
| Recombinant protein | Elution time (min) | % main peak |
|---|---|---|
| P. pastoris DBL4-7G8 | 33.2 | 52 |
| P. pastoris DBL5-7G8 | 30.5 | 97 |
| pET28a DBL4-7G8 | 33.6 | 62 |
| pET28a DBL4-FCR3 | 33.7 | 68 |
| pET28a DBL5-7G8 | 33.0 | 90 |
| pET28a DBL5-FCR3 | 32.2 | 99.2 |
| pET28b DBL4-7G8 | 33.5 | 38 |
| pET28b DBL4-FCR3 | 34.0 and 35.1 | 36 and 28 |
| pET28b DBL5-7G8 | 30.6 | 98 |
| pET28b DBL5-FCR3 | 33.7 | 73 |
| Baculovirus DBL4-7G8 | 32.7 and 33.0 | 52 and 31 |
| Baculovirus DBL4-FCR3 | 33.1 | 57 |
| Baculovirus DBL5-7G8 | 31.7 | 93 |
| Baculovirus DBL5-FCR3 | 32.9 | 86 |