Table 4.
Expected molecular mass versus molecular mass observed by MS analysis
| Recombinant protein | Molecular mass (Da) |
|
|---|---|---|
| Expected | Observed by MS | |
| P. pastoris DBL4-7G8 | 48,700 | 49,330 |
| P. pastoris DBL5-7G8 | 35,700 | 36,842 |
| pET28a DBL4-7G8 | 50,766 | 51,346 |
| pET28a DBL4-FCR3 | 50,058 | 50,554 and 50,779 |
| pET28a DBL5-7G8 | 35,183 | 37,935 and 75,197 (dimer) |
| pET28a DBL5-FCR3 | 35,000 | 35,760 |
| pET28b DBL4-7G8 | 48,500 | Peak 1, 25,146; peak 2, 50,135 |
| pET28b DBL4-FCR3 | 47,700 | Peak 1, 49,511; peak 2, 49,680 |
| pET28b DBL5-7G8 | 34,800 | 36,933 |
| pET28b DBL5-FCR3 | 32,900 | 34,905 |
| Baculovirus DBL4-7G8 | 50,601 | 54,390 |
| Baculovirus DBL4-FCR3 | 49,893 | 54,295 |
| Baculovirus DBL5-7G8 | 36,978 | 38,636 |
| Baculovirus DBL5-FCR3 | 34,324 | 35,926 |