Fig 1.

The FE6-C3 mutant contains an insertion in the HCL1 ORF. (A) Schematic of the HCL1 genomic locus showing the site of the TDNA insertion in the hcl1 mutant, the region of the HCL1 locus that was used to generate the complementation construct, and the region of the HCL1 gene that was used to probe transcript levels. Tick marks are 1 kb apart. (B and C) Total RNA was isolated from wild-type (WT), the hcl1 mutant, and the complemented strain (hcl1+HCL1) grown in broth culture at 37°C. HCL1 mRNA levels were monitored by quantitative RT-PCR (B) and Northern blot analysis (C). Primers for qRT-PCR analysis are described in Materials and Methods and located in the coding sequence of HCL1.