Fig 5.

Hcl1 is required for maintenance of neutral pH in culture. Four-day cultures of wild-type H. capsulatum (WT), the hcl1 mutant, and the complemented strain (hcl1+HCL1) were pelleted, washed, resuspended in PBS, and diluted into 30 ml of unbuffered pH-HMM at OD₆₀₀ of 2.0. The pH of the culture medium was measured at the indicated time points. The average of three pH measurements ± the standard deviation is shown from one representative experiment. In most cases, the standard deviation is negligible and is obscured by strain symbols.