Table 2.
Transposon mutants with reduced NaCl-induced colistin resistance
| Function(s) and strain | Insertion site | Locus tagb | Gene annotation |
|---|---|---|---|
| Phosphate metabolism | |||
| 2F12 | Intergenic | A1S_2445 | High-affinity phosphate transport protein (PstB) |
| 2F12 | Intergenic | A1S_2444 | Putative periplasmic protease |
| 19H7 | Gene | A1S_3030 | Phosphate-inducible protein, PhoH-like |
| 22F11 | Gene | A1S_0462 | Putative phosphatase |
| Envelope biogenesis | |||
| 6D2 | Gene | A1S_2982 | YidD |
| 11E4 | Gene | A1S_2250 | Zn-dependent protease with chaperone function |
| 20A5 | Intergenic | A1S_3424 | Putative lipoprotein-34 precursor (NlpB) |
| 5A7 | Gene | A1S_0430 | Putative glycosyltransferase (LpsB) |
| 23E5 | Gene | A1S_1030 | DNA-binding ATP-dependent protease La |
| 19C4 | Gene | A1S_0499 | Putative Fe-S cluster redox enzyme (rRNA large-subunit methyltransferase N) |
| RNA synthesis, processing, modification, and degradation | |||
| 20A5 | Intergenic | A1S_3425 | Phosphoribosylaminoimidazole-succinocarboxamide |
| 18G3a | Intergenic | A1S_0531 | Putative GTPase |
| 18G3a | Intergenic | A1S_0532 | Oligoribonuclease |
| 19C4 | Gene | A1S_0499 | Putative Fe-S cluster redox enzyme (rRNA large-subunit methyltransferase N) |
| 6D2 | Gene | A1S_2982 | YidD |
| Amino acid transport and metabolism (glutamate, aspartate, alanine), urea cycle | |||
| 22E8 | Gene | A1S_3185 | Glutamate synthase subunit alpha |
| 17H6 | Intergenic | A1S_1142 | Aspartate kinase |
| 17H6 | Intergenic | A1S_1143 | Hypothetical protein |
| 20B10 | Gene | A1S_2793 | Putative amino acid transport protein |
| 41C12a | Gene | A1S_2454 | Diaminobutyrate-2-oxoglutarate transaminase |
| 20A2 | Intergenic | A1S_2023 | Hypothetical protein |
| 20A2 | Intergenic | A1S_2024 | Glutamate 5-kinase |
| 20A1 | Gene | A1S_3025 | Malate dehydrogenase |
| TCA cycle | |||
| 31A2 | Gene | A1S_2710 | Citrate synthase |
| Cofactor biosynthesis | |||
| 14G3a | Gene | A1S_0807 | 8-Amino-7-oxononanoate synthase |
| Multidrug efflux system | |||
| 18D2 | Gene | A1S_0909 | Multidrug resistance protein B |
| 20A11 | Intergenic | A1S_1231 | Major facilitator superfamily transporter |
| 20A11 | Intergenic | A1S_1232 | EsvB |
| Lipid metabolism | |||
| 3H3 | Intergenic | A1S_2842 | Putative acetyl-CoA acetyltransferase |
| 20B2a | Intergenic | A1S_3159 | Lipase chaperone |
| 20B2a | Intergenic | A1S_3160 | Lipase |
| Phage | |||
| 37A5a | Intergenic | A1S_1585 | Putative replicative DNA helicase |
| 37A5a | Intergenic | A1S_1586 | EsvKI |
a
Mutant that is more resistant to colistin.
b
Locus tags refer to the NCBI reference sequence NC_009085.