Table 1.
Strain | Genotype or descriptiona | Reference or source |
---|---|---|
E. coli | ||
XL1 Blue | General cloning strain; recA1 endA1 gyrA96thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZM15::Tn10] Tetr | Stratagene |
C. jejuni | ||
81-176 | Serotype HS: 23, 26 | S. A. Thompson |
81-176 eptC | 81-176 cj0256 Camr | 7 |
81-176 eptC eptC+ | 81-176 cj0256 astA::cj0256+ Camr Kanr | 7 |
81-176 waaF | 81-176 waaF Kanr | This study |
81-176 eptC waaF | 81-176 cj0256 waaF Camr Kanr | This study |
Antibiotics were used at the following concentrations: for E. coli cultures, 100 μg/ml ampicillin (Amp), 30 μg/ml kanamycin (Kan), 10 μg/ml tetracycline (Tet); for C. jejuni cultures, 25 μg/ml chloramphenicol (Cam) and 50 μg/ml kanamycin.