Table 1.
Serum chemistry analysis of wild-type and IL-6 KO mice infected ID with 104 CFU LVSa
| Analyte | Concn of analyte found in mouse strain on: |
|||||
|---|---|---|---|---|---|---|
| Day 0 (naïve) |
Day 3 |
Day 7 |
||||
| WT | IL-6 KO | WT | IL-6 KO | WT | IL-6 KO | |
| Glucose | 328 (321–475) | 472 (204–504) | 148* (68–217) | 364* (228–460) | 272 (180–404) | 179 (124–288) |
| Cholesterol | 72 (56–88) | 66 (64–75) | 112 (91–146) | 72 (52–88) | 76 (72–100) | 60 (54–74) |
| Triglycerides | 136 (101–148) | 208 (169–272) | 84 (52–131) | 128 (94–176) | 153 (132–178) | 86 (54–110) |
| Sodium | 146 (140–162) | 160 (148–161) | 156 (148–162) | 156 (143–162) | 146 (137–159) | 155 (149–161) |
| Chloride | 111 (107–134) | 133 (112–134) | 126 (111–138) | 125 (106–138) | 113 (108–132) | 126 (116–133) |
| Magnesium | 1.0 (0.35–1.77) | 0.48 (0.43–1.42) | 0.8 (0.48–1.96) | 0.97 (0.48–1.46) | 1.86 (0.54–2.05) | 1.5 (0.56–2.07) |
| Alkaline phosphatase | 89 (78–150) | 86.5 (80–104) | 33* (20–56) | 67* (59–196) | 31 (24–37) | 86 (68–102) |
| ALT | 83 (50–207) | 56 (48–177) | 87 (56–290) | 133 (76–496) | 279* (181–336) | 816* (414–1,005) |
| AST | 287 (198–1,194) | 248 (204–921) | 449 (180–1,230) | 401 (232–1,352) | 644* (473–904) | 1,703* (626–1,962) |
| Amylase | 1,134 (961–1,316) | 1,408 (1,324–2,248) | 671* (516–816) | 1,043* (554–5,834) | 1,220 (956–1,692) | 1,276 (900–1,956) |
| Creatinine kinase | 2,609 (1,219–3,948) | 3,628** | 2,021 (2,008–3,188) | 3572 (878–5,219) | 1,004 (239–1,770) | 4,458 (3,112–5,804) |
| LDH | 864 (684–949) | 816 (588–828) | 1,144 (624–2,331) | 864 (828–2,335) | 1,450 (1,440–1,646) | 3,867 (3,423–4,311) |
| Uric acid | 5 (4.5–8.7) | 9 (5.9–9.6) | 6 (4–7.2) | 7.9 (6–9.2) | 6.8 (6.6–12.4) | 6.5 (6–9.9) |
Groups of six WT and six IL-6 KO mice were infected i.d. with 104 CFU LVS. Three mice from each group, as well as naive mice, were sacrificed at day 3 and day 7 after infection. Equivalent amounts of blood from each mouse were collected and pooled, sera were isolated, and sera samples were analyzed by the Department of Laboratory Medicine, Clinical Center, NIH. Data are presented as the median (range) for each analyte, in pg/ml, using all combined results from four independent experiments for the naïve groups and up to 7 for the infected groups. *, values from the samples from LVS-infected WT and IL-6 KO mice were significantly different from each other based on ANOVA; **, only 1 sample was available. Units for sodium, chloride, and magnesium values are mmol/liter. Units for glucose, cholesterol, triglycerides, and uric acid are mg/dl. Units for alkaline phosphatase, ALT, AST, amylase, creatinine kinase, and LDH are units/liter.