Table 2.
CBC analyses of whole blood from wild-type and IL-6 KO mice infected i.d. with 104 CFU LVSa
| CBC parameter | Composition of whole blood in mouse strain onb: |
|||||
|---|---|---|---|---|---|---|
| Day 0 (naïve) |
Day 3 |
Day 7 |
||||
| WT | IL-6 KO | WT | IL-6 KO | WT | IL-6 KO | |
| WBC | 5.7 (5.4–6.0) | 8.8 (7.6–14.4) | 4.7 (4.2–5.2) | 4.2 (3.6–4.8) | 9 (7.04–12.6) | 9.1 (7.6–22.1) |
| RBC | 8.5 (7.3–9.8) | 7.8 (6.5–12.4) | 10.1 (7.0–13.2) | 6.8 (5.4–10.9) | 7.3 (4.6–8.2) | 5.8 (4.3–9.3) |
| Hemoglobin | 13.4 (11.6–15.2) | 12.4 (10.4–15.2) | 15.8 (11.6–20) | 10.8 (8.8–16.3) | 10.8 (7.1–12.4) | 9 (6.8–14.4) |
| Hematocrit (PCV) | 37.6 (31.6–43.6) | 34.4 (28–50.3) | 42.6 (30–55.2) | 28.4 (22.8–47.9) | 32.5 (21.4–36) | 25 (19.5–39.2) |
| MCV | 43.6 (43.5–44.3) | 43.4 (40.6–44.2) | 42.1 (41.9–42.3) | 42.1 (41.9–44.1) | 44.1 (42.2–46.4) | 43.4 (42.2–45) |
| Platelets | 1162 (1,056–1,268) | 972 (732–1,108) | 946 (888–1,004) | 524 (236–576 | 668* (419–769) | 216* (104–304) |
| PMNs | 21.7 (21.3–22.1) | 8.2 (5.9–21.2) | 44 (28.1–59.8) | 30.4 (27.8–33.2) | 18.2 (14.5–28.3) | 17 (6.4–32.3) |
| Lymphocytes | 50.1 (48.4–51.8) | 80.3 (65.6–82) | 16.7 (16.1–17.3) | 28.3 (18.7–37.1) | 20.4 (14.6–46.1) | 17.3 (13.9–54.2) |
| Monocytes | 19.6 (16.1–23) | 4.4 (2.6–9.4) | 27.8 (15.2–40.3) | 36.9 (15.8–40.1) | 39.1 (20.1–46.4) | 43.5 (31.5–51) |
| Eosinophils | 2.3 (0.5–4.2) | 0.8 (0.5–6.5) | 0.9 (0.2–1.5) | 1.3 (0.8–7.5) | 0.7 (0.5–4.8) | 0.7 (0.1–3.6) |
| Basophils | 6.3 (5.8–6.8) | 5.4 (2.8–8.7) | 10.8 (7.4–14.1) | 6.4 (6.2–9.5 | 15.6 (10.6–18.6) | 11.7 (7.8–17.4) |
Groups of six WT and six IL-6 KO mice were infected i.d. with 104 CFU LVS. Three mice from each group, as well as naive mice, were sacrificed at day 3 and day 7 after infection. Equivalent amounts of blood from each mouse were collected and pooled, sera were isolated, and sera samples were analyzed by the Department of Laboratory Medicine, Clinical Center, NIH. Data are presented as the median (range) for each analyte, using all combined results from four independent experiments for the naïve groups and from up to seven independent experiments for the infected groups. *, values from the sera samples from LVS-infected WT and IL-6 KO mice were significantly different from each other, based on ANOVA. WBC, white blood cells; RBC, red blood cells; PCV, packed cell volume; MCV, mean corpuscular volume; PMNs, polymorphonuclear leukocytes.
Units for WBC and platelets values are K/μl. Unit for RBC is millions/μl. Unit for hemoglobin is g/dl. Units for hematocrit, PMNs, lymphocytes, monocytes, eosinophils, and basophils are percentages. Unit for MCV is fl.