Table 3.
Splenocyte subpopulations in mice infected with 104 CFU LVS i.d.a
| Cell type and marker(s) | % of cells with indicated marker(s) in mouse strain on: |
|||
|---|---|---|---|---|
| Day 1 |
Day 6 |
|||
| WT | IL-6 KO | WT | IL-6 KO | |
| B cells | ||||
| CD19+ B220+ | 33.3 ± 4.4 | 33.7 ± 2.5 | 48.5 ± 1.3 | 54.5 ± 4.7 |
| T cells | ||||
| Total α/β TCR+ | 52.3 ± 3.2 | 50.3 ± 6 | 31 ± 2.5 | 31.4 ± 3.3 |
| CD4+ | 31.6 ± 3 | 28.1 ± 4 | 17.3 ± 4.3 | 14.7 ± 2.5 |
| CD8+ | 23.6 ± 10.1 | 20.3 ± 1.9 | 13.5 ± 3.6 | 15.0 ± 3 |
| CD4− CD8− | 2 ± 0.8 | 2.4 ± 2 | 2.2 ± 0.3 | 1.5 ± 0.4 |
| Macrophages | ||||
| CD11b+ CD11c− Gr-1− | 2.9 ± 0.8 | 4 ± 0.6 | 3.4 ± 0.5 | 2.6 ± 1.1 |
| Dendritic cells | ||||
| CD11c+ | 0.7 ± 0.1 | 0.9 ± 1.9 | 2.7 ± 1.5 | 3.4 ± 2 |
| Neutrophils | ||||
| Gr-1hi CD11bhi | 6.0 ± 4.4 | 3.9 ± 2.4 | 4.6 ± 2.3 | 2.5 ± 1.5 |
| NK cells | ||||
| NK1.1+ | 3.6 ± 1.8 | 3.7 ± 1.4 | 3.4 ± 0.5 | 2.3 ± 0.5 |
a
Wild-type C57BL/6 and IL-6KO mice were infected i.d. with 104 CFU LVS. Groups of three mice from each group were sacrificed on days 1 and 6 after infection, spleens were harvested and pooled, and splenocytes were prepared for analyses by multiparameter flow cytometry. Cells were analyzed on an LSR II cytometer using appropriate single-color compensation and isotype controls; only live nonaggregated cells were included in the analyses. Data represent means ± standard deviations of data combined from three independent experiments.