Fig 4.

Restriction map of plasmid pSE11 and its derivatives, used to complement XJ3 and to construct chromosomal knockout alleles. Plasmid pSE11 and its pSE21, pSE22, and pSE23 derivatives, constructed as described in Materials and Methods, are shown on the left, and their ability to complement the NADI phenotype of the Cu-unresponsive ΔccoA derivative XJ3 is shown on the right. When appropriate, R. capsulatus strains SE17, SE18, and SE19, as well as SE20, SE21, and SE22, carrying appropriate ccmA, ccmB, and ccmAB chromosomal knockout alleles, respectively, are indicated under the related plasmids. See the text for information on the ORFs annotated RCC01783, RCC01784, RCC01785, and RCC01786 in pSE11. BsE, BsB, X, XB, and K correspond to the restriction endonuclease sites for the BstEII, BstBI, XhoI, XbaI, and KpnI enzymes, respectively. A prime symbol indicates a partial gene.