Table 1.
Strains and plasmids used in this work
| Strain or plasmid | Description | Phenotype | Reference |
|---|---|---|---|
| Strains | |||
| E. colistrains | |||
| HB101 | F− Δ(gpt-proA)62 leuB6 supE44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1recA13 | Strr | 16 |
| XL1-Blue | F′::Tn10 proA+B+ lacIqZΔM15 recA1 endA1 gyrA96 (Nalr) thi hsdR17(rK− mK+) supE44 relA1 lac | Ampr | Stratagene |
| R. capsulatus strains | |||
| MT1131 | crtD121 | Wild type (NADI+), Rifr | 38 |
| Y262 | GTA overproducer | 18 | |
| MR1 | ccoO1 | NADI− on MPYE | 9 |
| GK2 | ccoO2 | NADI− on MPYE | 9 |
| SE8 | Δ(ccoA::spe) | NADI− on MPYE, NADIslow on MedA | 11 |
| GK32 | Δ(ccoNO::kan) | NADI− | 9 |
| MD12 | Δ(ccmF::spe) | Sper | 39 |
| MT-RBC1 | Δ(petABC::spe) | Sper | 12 |
| XJ3 | ccmA3 Δ(ccoA::spe) | Sper NADI− (+ or − Cu) Psslow | This work |
| XJ11 | ccmF11 Δ(ccoA::spe) | Sper NADI− (+ or − Cu) Psslow | This work |
| SE17 | Δ(ccmA::kan) Δ(ccoA::spe) | Kanr Sper NADI− Ps− | This work |
| SE18 | Δ(ccmB::kan) Δ(ccoA::spe) | Kanr Sper NADI− Ps− | This work |
| SE19 | Δ(ccmAB::kan) Δ(ccoA::spe) | Kanr Sper NADI− Ps− | This work |
| SE20 | Δ(ccmA::kan) | Kanr NADI− Ps− | This work |
| SE21 | Δ(ccmB::kan) | Kanr NADI− Ps− | This work |
| SE22 | Δ(ccmAB::kan) | Kanr NADI− Ps− | This work |
| Plasmids | |||
| pRK2013 | Conjugation helper | Kanr | 40 |
| pRK404 | Broad-host-range vector | Tetr | 40 |
| pRK415 | Broad-host-range vector | Tetr | 40 |
| pBluescript II KS(+) | Ampr | Stratagene | |
| pCW25 | ccoNOQP-ccoGHIS in pRK415 | Tetr | 23 |
| pMA117 | Carries nonpolar kan resistance cassette | Kanr | 17 |
| pSE3 | ccoA | Tetr | 11 |
| pSE10 | 7.6-kb chromosomal BamHI fragment on pRK415 | Tetr | This work |
| pSE11 | 4,912 bp deleted between XbaI and BstBI | Tetr | This work |
| pSE12 | 2,714 bp deleted between BstBI and KpnI | Tetr | This work |
| pSE14 | 2,389-bp XbaI-KpnI fragment containing ccmABcloned into XbaI and KpnI sites of pBluescript II KS(+) | Ampr | This work |
| pSE15 | kan cassette inserted at the BstEII site of ccmA | Ampr Kanr | This work |
| pSE16 | kan cassette inserted at the XhoI site of ccmB | Ampr Kanr | This work |
| pSE17 | 746 bp between BstEII and XhoI sites replaced by kan cassette | Ampr Kanr | This work |
| pSE21 | KpnI-XbaI fragment with Δ(ccmA::kan) in pRK415 | Kanr Tetr | This work |
| pSE22 | KpnI-XbaI fragment with Δ(ccmB::kan) in pRK415 | Kanr Tetr | This work |
| pSE23 | KpnI-XbaI fragment with Δ(ccmAB::kan) in pRK415 | Kanr Tetr | This work |
| pCS1582 | Strep-ccmF ccmH ccmI-FLAG+ | Tetr | 41 |
| pSVEN | ccmI | Tetr | 29 |
| pYZ4 | ccmF::spe ccmH | Sper Tetr | 39 |
| pYZ12 | ccmF ccmH::spe | Sper Tetr | 39 |