Table 2.
Metal contents of wild-type strain (MT1131), ΔccoA strain (SE8), and Cu-unresponsive mutants XJ3 and XJ11 as determined by ICP-DRC-MS
| Strain | Presence of Cu2+ (5 μM)a | Metal content (%)b |
Cu/Mn ratio | |
|---|---|---|---|---|
| Cu | Mn | |||
| Wild type (MT1131) | − | 100 | 100 | 1.00 |
| + | 330 | 107 | 3.08 | |
| ΔccoA strain (SE8) | − | 77 | 110 | 0.70 |
| + | 292 | 112 | 2.60 | |
| XJ3 | − | 89 | 110 | 0.81 |
| + | 312 | 122 | 2.56 | |
| XJ11 | − | 92 | 129 | 0.71 |
| + | 134 | 126 | 1.06 | |
| Δcbb3 strain (GK32) | − | 94 | 140 | 0.67 |
| + | 347 | 140 | 2.47 | |
a
− and +, absence and presence, respectively, of 5 μM Cu2+ supplement during growth by respiration in MPYE medium as described in Materials and Methods.
b
See Materials and Methods for details on the determination of the amounts of Cu and Mn in cells. A content of 100% corresponds to 11.4 and 6.7 μg of Cu and Mn, respectively, per gram of lyophilized cells in all cases.