Table 1.
Plasmids
| Plasmid | Genotype | Reference |
|---|---|---|
| pLH25 Kmr (pET28a) | Expresses the A. brasilense GlnB | 25 |
| pMSA4 Kmr (pET28a) | Expresses the A. brasilense GlnZ | 26 |
| pLHPETDraT Kmr (pET28a) | Expresses the N-terminally His-tagged A. brasilense DraT | 27 |
| pLHDK5pII Ampr (pDK5) | Expresses the A. brasilense GlnB | 25 |
| pMSA4 Q42HS52VS54D Kmr (pET28a) | Expresses the A. brasilense GlnZ variant Q42HS52VS54D | This work |
| pMSA4 S52VS54D Kmr (pET28a) | Expresses the A. brasilense GlnZ variant S52VS54D | This work |
| pLH25PETGlnB H42Q Kmr (pET28a) | Expresses the A. brasilense GlnB variant H42Q | This work |
| pGlnBΔ42–54 Kmr (pET28a) | Expresses the A. brasilense GlnB carrying a T-loop deletion (Δ42-54) | This work |
| pGlnDHis Kmr (pET28a) | Expresses the N-terminally His-tagged A. brasilense GlnD | 28 |