Fig 2.

Inactivation of oprF and oprI increases P. aeruginosa OMV production. (A) Fold change in OMV production by the P. aeruginosa oprF, oprL, and oprI mutants. Bacteria were grown with shaking (250 rpm) at 37°C to an OD₆₀₀ of ∼3.5, and OMVs were quantified by measuring OMV lipid. All lipid measurements were normalized to cell number. For each replicate, the fold change in OMV production was calculated by dividing mutant lipid levels by wt lipid levels. The dotted line represents no change in OMV production. Error bars represent standard errors of the means (n ≥ 4). (B) Complementation of the oprF and oprI mutants. The fold change in OMV production by the oprF and oprI mutants carrying either vector alone (pEX1.8) or the complementation plasmids (pEX1.8-oprF or pEX1.8-oprI) is shown. Bacteria were grown with shaking (250 rpm) at 37°C to an OD₆₀₀ of ∼3.5 with 500 μM IPTG. OMVs were quantified and compared to those of wt P. aeruginosa carrying pEX1.8 as described for panel A. Error bars represent standard errors of the means (n ≥ 4).