Fig 3.

OMVs from the P. aeruginosa oprF mutant are not enriched for cytoplasmic membrane proteins. Two biological replicates displaying the proportion of protein abundance from each cellular compartment in wt P. aeruginosa OMVs and P. aeruginosa oprF mutant OMVs. The protein abundance of each compartment was estimated by dividing the sum of APEX scores of proteins identified in each compartment by the total APEX score for each sample. Protein localization predictions were obtained from the Pseudomonas Genome Database. In wt PA14 samples, 159 (first replicate) and 533 (second replicate) proteins were identified. In the oprF mutant samples, 504 (first replicate) and 1,140 (second replicate) proteins were identified.