Table 2.

Primer sequences

Name	Sequencea
oprL-flanking regions
oprL-P1	CCGGATCCGAGAAGCTCACCGGTATCAAG
oprL-P2	GTGCTTGGGCATAACGACTTCCATGTAACTCCTAATGAACCC
oprL-P3	GAAGTCGTTATGCCCAAGCAC
oprL-P4	CAGGATCCGTACTGGGAAATGACCTGCTG
oprI-flanking regions
oprI-P1	5′-CCGGATCCAGGTACTCCAGGTTCAGCCAC
oprI-P2	5′-GTTTTCAACAGGTCGTGAGACCGGTGGACATTTCCATAACAGCAATC
oprI-P3	5′-GGTCTCACGACCTGTTGAAAAC-3′
oprI-P4	5′-CCGGATCC AGGTGATCAAGGCCAAGTAC-3′
pqsH-flanking regions
pqsH-P1	5′-CTGAATTCCTTGTCCTGCAGGTCGATATC-3′
pqsH-P2	5′-CATCGCCGAACTCGAAAACAGGATAAGAACGGTCATCCGTTGC-3′
pqsH-P3	5′-GCAACGGATGACCGTTCTTATCCTGTTTTCGAGTTCGGCGATG-3′
pqsH-P4	5′-CTTCTAGAGATTGCTACAGGTAGCGAGG-3′
Complementation
oprF-for	5′-CTAACTGACCATCAAGATGGG-3′
oprF-rev	5′-CCCAAGCTTTTTTCCTTAGAGGCTCA-3′
oprI-for	5′-CGGAATTCGTCCACCTTAAGGGGAAC-3′
oprI--rev	5′-CCCAAGCTTCAGGTCGTGAGACCTAT-3′

^aUnderlined sequences represent recognition sites for restriction endonucleases.