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Absolute quantification of yqjG and spoIIIJ transcripts at pH 7.5 and 10.5 by qPCRa
| pH | Level (copies/ng total RNA) of: |
|
|---|---|---|
| yqjG | spoIIIJ | |
| 7.5 | (1.21 ± 0.17) × 104 | (3.05 ± 0.63) × 104 |
| 10.5 | (0.44 ± 0.09) × 104 | (3.48 ± 0.23) × 104 |
The recA gene was used as an internal standard gene, and it had values of (2.02 ± 0.3) × 104 and (2.14 ± 0.18) × 104 copies/ng total RNA at pH 7.5 and 10.5, respectively.
