Table 1.
Strains and plasmids used for this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| 168 | trpC2 | Laboratory stock |
| TF10 | trpC2 ccpA (Cmr) | 33 |
| OAM632 | trpC2 hprK (Pmr) | This study |
| OAM588 | trpC2 clpC (Tcr) | 26 |
| CU741T | trpC2 leuC7 tnrA (Cmr) | 27 |
| 168 glnA14 | trpC2 glnA (Spr) | 29 |
| OAM415 | trpC2 amyE::degU-lacZ U-WT (Cmr) | 26 |
| OAM633 | trpC2 amyE::degU-lacZ U-del11 (Cmr) | This study |
| OAM634 | trpC2 amyE::degU-lacZ U-M15 (Cmr) | This study |
| OAM516 | trpC2 amyE::degU-lacZ U-del3 (Cmr) | 26 |
| OAM636 | trpC2 amyE::degU-lacZ U-P2 (Cmr) | This study |
| OAM637 | trpC2 amyE::degU-lacZ U-P2 (Cmr) glnA(Spr) | This study |
| OAM638 | trpC2 amyE::degU-lacZ U-P2 (Cmr) glnA(Spr) tnrA(Cmr::Kmr) | This study |
| OAM639 | trpC2 amyE::degU-lacZ U-P2 (Cmr::Tcr) glnA(Spr) ccpA(Cmr) | This study |
| OAM420 | trpC2 amyE::degU-lacZ U-WT (Cmr) clpC(Tcr) | 26 |
| OAM416 | trpC2 amyE::degU-lacZ U-WT (Cmr) degU(Kmr) | 26 |
| OAM640 | trpC2 thrC::degU-lacZ U-WT (Spr) | This study |
| OAM641 | trpC2 thrC::degU-lacZ U-WT (Spr) ccpA (Cmr) | This study |
| OAM642 | trpC2 thrC::degU-lacZ U-WT (Spr) hprK (Pmr) | This study |
| OAM643 | trpC2 thrC::degU-lacZ U-WT (Spr) tnrA (Cmr) | This study |
| OAM371 | trpC2 amyE::sacB-lacZ S-WT (Cmr) | 20 |
| OAM372 | trpC2 amyE::sacB-lacZ S-WT (Cmr) degU(Kmr) | 20 |
| OAM367 | trpC2 amyE::bpr-lacZ B-WT (Cmr) | 23 |
| OAM368 | trpC2 amyE::bpr-lacZ B-WT (Cmr) degU(Kmr) | 23 |
| OAM644 | trpC2 amyE::PclpC-bgaB (Cmr::Tcr) | This study |
| OAM645 | trpC2 amyE::PclpC-bgaB (Cmr::Tcr) ccpA (Cmr) | This study |
| OAM646 | trpC2 amyE::PclpC-bgaB (Cmr::Tcr) hprK(Pmr) | This study |
| Plasmids | ||
| pDG1729 | Insertion vector for thrC, spectinomycin and erythromycin resistance genes, and lacZ | 34 |
| pDG1729-degU | pDG1729 carrying a promoter region of degU (positions −184 to +56) | 26 |
| pIS284 | Insertion vector for amyE, chloramphenicol resistance gene, and lacZ | 20 |
| pIS284-degU-del11 | pIS284 carrying a promoter region of degU (positions −151 to +56) | This study |
| pIS284-degU-M15 | pIS284 carrying a promoter region of degU (positions −151 to +56), with CG-to-TT mutation (positions −135 and −136) | This study |
| pIS284-degU-P2 | pIS284 carrying a promoter region of degU (positions −184 to −34) | This study |
| pPhl2 | Phleomycin resistance | 35 |
| pPhl2-hprK | pPhl2 carrying part of hprK | This study |
| ECE75 | Ampicillin resistance; Cmr::Tcr | BGSC |
| ECE73 | Ampicillin resistance; Cmr::Kmr | M. Steinmetz |
| His-CcpA | pQE30-based vector for production of CcpA-His | 36 |
| His-MecA | pQE8-based vector for production of MecA-His | This study |