Table 3.
MICs of amphotericin B, itraconazole, ketoconazole, fluconazole, sulfamethoxazole, sulfamethoxazole-trimethoprim, and terbinafine for 21 Paracoccidioides brasiliensis isolates in different culture media at 37°C after 15 days of incubation
| Isolate | MIC (mg/liter) |
||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amphotericin B |
Itraconazole |
Ketoconazole |
Fluconazole |
Sulfamethoxazole |
Sulfamethoxazole-trimethoprim |
Terbinafine |
|||||||||||||||
| RPMI | MMH | MVM | RPMI | MMH | MVM | RPMI | MMH | MVM | RPMI | MMH | MVM | RPMI | MMH | MVM | RPMI | MMH | MVM | RPMI | MMH | MVM | |
| Ed01 | 1.0 | 0.25 | 1.0 | 0.015 | 0.0039 | 0.062 | 0.0039 | 0.0019 | 0.015 | 2.0 | 0.25 | 1.0 | 300 | 75 | 2.34 | 300 | 150 | 4.68 | 0.25 | 0.0078 | 0.25 |
| 1578 | 0.5 | 0.125 | 0.0078 | 0.031 | 0.0039 | 0.0039 | 0.0019 | 0.0019 | 0.0019 | 0.5 | 0.5 | 0.0078 | 300 | 37.5 | 1.17 | 75 | 37.5 | 1.17 | 0.25 | 0.25 | 0.0078 |
| Pb01 | 1.0 | 0.25 | — | 0.0078 | 0.0039 | — | 0.0019 | 0.0019 | — | 0.5 | 0.25 | — | 150 | 75 | — | 150 | 75 | — | 0.125 | 0.015 | — |
| Pb4 | 1.0 | 0.125 | 1.0 | 0.062 | 0.0039 | 0.25 | 0.031 | 0.0019 | 0.031 | 8.0 | 1.0 | 1.0 | 300 | 150 | 4.68 | 300 | 150 | 4.68 | 0.5 | 0.125 | 1.0 |
| Pb2 | 0.25 | 0.125 | 0.031 | 0.0078 | 0.0039 | 0.0078 | 0.0019 | 0.0019 | 0.0039 | 0.5 | 0.25 | 0.5 | 150 | 150 | 2.34 | 150 | 75 | 4.68 | 0.5 | 0.015 | 0.5 |
| Pb03 | 1.0 | 1.0 | 0.5 | 0.015 | 0.0039 | 0.25 | 0.015 | 0.0019 | 0.031 | 2.0 | 1.0 | 2.0 | 300 | 150 | 4.68 | 300 | 300 | 9.37 | 0.5 | 0.062 | 1.0 |
| Pb6 | 1.0 | 0.25 | 0.062 | 0.0078 | 0.0039 | 0.0078 | 0.0019 | 0.0019 | 0.0078 | 0.5 | 0.25 | 1.0 | 300 | 75 | 4.68 | 300 | 150 | 2.34 | 0.25 | 0.015 | 0.5 |
| Pb5 | 1.0 | 0.25 | 0.125 | 0.015 | 0.0039 | 0.0078 | 0.0078 | 0.0019 | 0.0039 | 1.0 | 0.25 | 1.0 | 300 | 75 | 18.75 | 300 | 75 | 9.37 | 0.5 | 0.031 | 0.5 |
| B339 | 1.0 | 0.125 | 0.031 | 0.0039 | 0.0039 | 0.0078 | 0.0019 | 0.0019 | 0.0078 | 0.5 | 1.0 | 1.0 | 150 | 150 | 9.37 | 300 | 150 | 9.37 | 0.25 | 0.031 | 0.5 |
| Útero | 1.0 | 0.25 | 1.0 | 0.0039 | 0.0039 | 0.015 | 0.0019 | 0.0019 | 0.0078 | 0.5 | 0.25 | 0.125 | 300 | 75 | 1.17 | 150 | 75 | 1.17 | 0.25 | 0.015 | 0.125 |
| Pb11 | 0.5 | 0.25 | 0.5 | 0.062 | 0.0039 | 0.015 | 0.031 | 0.0019 | 0.0078 | 8.0 | 0.5 | 0.5 | 150 | 9.37 | 1.17 | 150 | 18.75 | 1.17 | 0.5 | 0.031 | 0.5 |
| Pb13 | 0.25 | 0.125 | 1 | 0.0039 | 0.0039 | 0.015 | 0.0019 | 0.0019 | 0.0039 | 0.25 | 0.125 | 0.5 | 150 | 75 | 4.68 | 300 | 150 | 4.68 | 0.25 | 0.015 | 1.0 |
| Pb10 | 1.0 | 0.25 | 0.5 | 0.0078 | 0.0039 | 0.062 | 0.0019 | 0.0019 | 0.0078 | 2.0 | 0.125 | 0.5 | 150 | 75 | 2.34 | 150 | 75 | 4.68 | 0.5 | 0.015 | 0.5 |
| Pb14 | 2.0 | 0.125 | 2.0 | 0.0078 | 0.0039 | 0.0039 | 0.0019 | 0.0019 | 0.0019 | 0.5 | 0.125 | 0.25 | 300 | 150 | 4.68 | 150 | 150 | 4.68 | 0.25 | 0.015 | 1.0 |
| 63265 | 1.0 | 0.25 | 0.5 | 0.0078 | 0.0039 | 0.0039 | 0.0019 | 0.0019 | 0.0039 | 0.5 | 0.25 | 0.25 | 300 | 37.5 | 1.17 | 150 | 75 | 1.17 | 0.125 | 0.015 | 0.25 |
| Pb09 | 1.0 | 0.25 | 0.062 | 0.062 | 0.0039 | 0.0078 | 0.0039 | 0.0019 | 0.0078 | 1.0 | 0.5 | 1.0 | 300 | 150 | 18.75 | 300 | 300 | 9.37 | 0.5 | 0.015 | 1.0 |
| Penguin | 1.0 | 0.25 | 0.062 | 0.0039 | 0.0039 | 0.0078 | 0.0078 | 0.0019 | 0.0078 | 2.0 | 1.0 | 1.0 | 300 | 150 | 18.75 | 150 | 150 | 18.75 | 0.5 | 0.125 | 0.5 |
| Tatu | 1.0 | 0.25 | 0.5 | 0.0078 | 0.0039 | 0.0039 | 0.015 | 0.0019 | 0.0039 | 1.0 | 0.125 | 0.25 | 300 | 75 | 2.34 | 300 | 75 | 4.68 | 0.25 | 0.0078 | 0.5 |
| Pb9 | 2.0 | 0.25 | 1.0 | 0.015 | 0.0039 | 0.015 | 0.0039 | 0.0019 | 0.0039 | 1.0 | 0.062 | 0.25 | 300 | 75 | 1.17 | 150 | 37.5 | 2.34 | 0.25 | 0.25 | 0.5 |
| Pb8 | 1.0 | 0.25 | — | 0.062 | 0.0039 | — | 0.0039 | 0.0019 | — | 1.0 | 0.25 | — | 300 | 75 | — | 150 | 75 | — | 0.5 | 0.031 | — |
| Pb18 | 1.0 | 0.25 | 0.031 | 0.0039 | 0.0039 | 0.0078 | 0.0019 | 0.0019 | 0.0078 | 0.25 | 0.25 | 1.0 | 300 | 150 | 18.75 | 150 | 150 | 4.68 | 0.25 | 0.031 | 1.0 |
—, no growth detected. RPMI, RPMI 1640 medium; MMH, MMH medium; MVM, MVM medium.