Table 5.
Conditions employed in previous studies and in the present work for determination of MIC against Paracoccidioides species complex: a tentative protocol based on our present resultsa
| Conditions | Previous references | This work | Proposed protocol |
|---|---|---|---|
| Inoculum concn | 1 × 103 to 1 × 105 cells/ml (9, 15, 16, 18, 20, 21) | 1 × 103, 1 ×104, and 1 ×105 cells/ml | 1 × 105 cells/ml |
| Incubation temp | 35–37°C, room temp (6, 11, 12, 15–21, 23) | 35–37°C, room temp | 35–37°C |
| Media | RPMI, MMH, MVM (9, 23, 30) | RPMI,c MMH, MVM | MMH and MVMb |
| Reading time | 72 h to 8 days (15–19) | 7, 10, and 15 days | 15 days |
| Protocol | Macro- and microdilution (6, 15–18) | Microdilution | Microdilution |
a
RPMI, RPMI 1640 medium; MMH, MMH medium; MVM, MVM medium.
b
MVM is to be used when necessary to test sulfamethoxazole and the combination sulfamethoxazole-trimethoprim.
c
When employed at 37°C, mycelial morphology may be found.