Table 3.
Genotypic prediction of the tropism of the GenBank HIV-1 subtype CRF01-AE virus clones using a specific algorithm
| Genotypic tool | Virus | No. of clones with indicated phenotype |
Genotypic predictiona |
|||||
|---|---|---|---|---|---|---|---|---|
| R5 | R5X4/X4 | % Sen | % Spe | % PPV | % NPV | Concordance (%) | ||
| Combined NXT/S mutation, 11/25, and net charge rule for subtype CRF01-AE | R5 | 48 | 1 | 91 | 96 | 83 | 98 | 95 (κ = 0.84; P < 0.0001) |
| X4 | 2 | 10 | ||||||
a
Sen, sensitivity for detecting CXCR4-using viruses; Spe, specificity for detecting exclusive CCR5-using viruses; PPV, positive predictive value; NPV, negative predictive value; κ, kappa coefficient.