Examples of eight different amplicon sizes associated with eight different tandem-repeat copy numbers for MIN22. The amplicons from lanes 2 to 8 are from isolates of M. intracellulare, while that in lane 9 is from another MAC species (MAC “X”). The gel was performed with 2% agarose in 1× TAE buffer. Lane 1, 100-bp ladder; lane 2, strain 3068 (6.93 copies) (600 bp); lane 3, strain 1760 (5.9 copies) (550 bp); lane 4, strain 07-3369 (4.94 copies) (500 bp); lane 5, ATCC 13950T (3.93 copies) (450 bp); lane 6, strain 2788 (3.04 copies) (400 bp); lane 7, strain 0416 (2.3 copies) (340 bp); lane 8, strain 289-0316 (0.93 copies) (275 bp); lane 9, water isolate LH-Sw-11-2 (0.16 copy) (220 bp); lane 10, 100-bp ladder.