Table 2.

Analytical performance of enteric TaqMan Array Card^a

Organism	Linearityb R2 (efficiency, %)	LOD (equiv. no. of copies)c	Low- and high-concn CV (%) ford:		Accuracy (%)d
			Repeatability	Reproducibility	Sensitivitye	Specificity
Adenovirus	0.997 (99.7)	106 (100)	1.1, 1.7	2.6, 4.8	100	100
Astrovirus	0.999 (93.0)	5 × 106 (500)	2.2, 2.6	3.2, 5.3	95	100
Norovirus GII	0.998 (99.2)	5 × 106 (500)	1.9, 1.1	3.5, 5.3	97	100
Rotavirus	0.993 (98.4)	5 × 106 (500)	1.5, 1.8	4.2, 7.5	100	100
Sapovirus	0.994 (94.9)	5 × 106 (500)	1.8, 2.9	5.8, 7.9	97	100
EAEC
aaiC	0.995 (99.7)	105 (10)	1.4, 2.8	4.2, 6.6	100	100
aatA	0.998 (95.5)	105 (10)	2.0, 3.0	6.3, 7.9	98	100
STEC
stx1	0.999 (96.3)	105 (10)	0.9, 2.9	3.5, 5.8	100	100
stx2	0.999 (94.6)	105 (10)	0.9, 1.8	4.2, 5.5	100	100
EPEC
eae	0.998 (100.2)	105 (10)	1.2, 1.5	3.6, 4.9	100	100
bfpA	0.998 (96.7)	105 (10)	0.9, 1.3	4.2, 5.2	100	100
ETEC
LT	0.998 (95.8)	105 (10)	0.7, 1.3	2.5, 3.6	100	100
ST	0.998 (95.5)	105 (10)	1.0, 1.1	3.1, 6.2	100	100
EIEC/Shigella	0.997 (104.2)	105 (10)	1.2, 1.3	3.6, 5.2	100	100
Campylobacter	0.997 (95.9)	106 (100)	0.9, 2.4	4.9, 7.2	100	100
Salmonella	0.997 (100.9)	106 (100)	2.3, 2.1	7.2, 7.5	98	100
V. cholerae	0.997 (102.1)	106 (100)	1.1, 1.7	7.6, 8.4	100	100
C. difficile	1.000 (99.4)	106 (100)	2.2, 1.9	4.2, 5.1	97	100
Cryptosporidium	0.998 (100.4)	103 (0.1)	2.0, 2.9	3.6, 6.3	100	100
Giardia	0.998 (101.4)	103 (0.1)	1.7, 3.0	5.2, 4.7	100	100
E. histolytica	0.988 (93.0)	103 (0.1)	2.0, 3.6	6.8, 6.7	100	100
Ascaris	0.993 (90.6)	106 (100)	1.3, 2.5	4.5, 7.3	100	100
Trichuris	0.999 (94.6)	106 (100)	0.7, 0.7	3.6, 5.2	100	100
PhHV	0.998 (98.3)		3.1, 1.8	4.2, 3.5
MS2	0.999 (96.8)		1.4, 2.1	7.2, 8.4
Bacterial 16S rRNA gene	0.995 (91.0)		4.9, 4.2	5.1, 4.7

^aLinearity was tested with combined positive controls. LOD and precision (repeatability and reproducibility) were tested with pooled positive materials spiked into healthy donor stool. LOD is the number of copies per gram of stool that were 100% detectable with 10 distinct extractions/amplifications.

^bThe linearity range was 10 to 10⁶ copy numbers per reaction for all the targets.

^cLOD, copy number of an organism or artificial template per gram of stool; equiv. no. of copies, equivalent copy numbers per 1-μl volume of reaction mixture prior to extraction.

^dCoefficients of variance (CVs) at both low (LOD) and high spiked concentrations are shown. High-concentration spikes were 1,000-fold higher for viral targets and 100-fold higher for bacterial and parasitic targets than LOD. The same concentrations were applied to accuracy tests.

^eSensitivity was calculated on the total number of PCRs for each target.