Table 1.
Labeling antibody | Reactivity for capture antibody and antigen |
||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1031 |
1061 |
1101 |
1321 |
1401 |
1451 |
1631 |
1711 |
1771 |
1841 |
1881 |
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Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | Recombinant LukF | Culture supernatant LukF-P83 | Culture supernatant ATCC 25923 | |
1031 | 0 | (+) | 0 | 0 | 0 | 0 | 0 | + | 0 | 0 | (+) | 0 | (+) | + | 0 | (+) | ++ | (+) | 0 | 0 | 0 | 0 | 0 | (+) | + | ++ | (+) | + | ++ | (+) | 0 | (+) | (+) |
1061 | 0 | (+) | 0 | 0 | 0 | 0 | 0 | + | 0 | (+) | ++ | 0 | 0 | + | 0 | 0 | (+) | 0 | 0 | 0 | 0 | 0 | + | 0 | 0 | + | (+) | 0 | + | (+) | 0 | (+) | (+) |
1101 | 0 | + | 0 | 0 | (+) | 0 | 0 | (+) | (+) | 0 | ++ | 0 | 0 | (+) | +++ | + | ++ | 0 | 0 | 0 | 0 | 0 | + | 0 | + | ++ | 0 | ++ | ++ | ++ | 0 | (+) | 0 |
1321 | 0 | + | 0 | 0 | (+) | 0 | (+) | ++ | 0 | 0 | (+) | 0 | 0 | (+) | 0 | ++ | +++ | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ++ | +++ | (+) | +++ | +++ | 0 | 0 | + | 0 |
1401 | + | ++ | 0 | 0 | + | 0 | 0 | + | ++ | 0 | + | 0 | 0 | (+) | ++++ | ++ | +++ | + | 0 | (+) | 0 | 0 | (+) | 0 | ++ | +++ | + | +++ | +++ | +++ | 0 | ++ | 0 |
1451 | (+) | ++ | 0 | 0 | 0 | 0 | ++ | +++ | (+) | +++ | +++ | 0 | + | ++ | ++ | 0 | ++ | 0 | 0 | (+) | 0 | ++ | +++ | 0 | (+) | ++ | (+) | + | +++ | (+) | 0 | + | 0 |
1631 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | (+) | (+) | 0 | 0 | 0 | 0 | (+) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | (+) | (+) | 0 | (+) | (+) | 0 | (+) | (+) |
1711 | 0 | ++ | 0 | 0 | + | 0 | (+) | ++ | 0 | 0 | + | 0 | 0 | + | 0 | +++ | +++ | (+) | 0 | 0 | 0 | 0 | (+) | 0 | +++ | +++ | (+) | +++ | ++++ | (+) | + | ++ | ++ |
1771 | + | ++ | 0 | 0 | 0 | 0 | ++ | +++ | (+) | +++ | ++++ | 0 | + | +++ | ++ | (+) | ++ | (+) | 0 | (+) | 0 | ++ | +++ | 0 | + | ++ | (+) | + | +++ | (+) | 0 | ++ | (+) |
1841 | + | +++ | 0 | 0 | 0 | 0 | +++ | ++++ | ++ | +++ | ++++ | 0 | ++ | +++ | +++ | 0 | ++ | 0 | 0 | (+) | 0 | +++ | ++++ | 0 | + | ++ | 0 | + | +++ | + | 0 | ++ | 0 |
1881 | 0 | (+) | (+) | 0 | 0 | 0 | 0 | (+) | 0 | 0 | (+) | 0 | 0 | (+) | 0 | 0 | (+) | (+) | 0 | (+) | (+) | 0 | (+) | (+) | 0 | (+) | (+) | 0 | (+) | (+) | 0 | (+) | (+) |
0, No reactivity; (+) to +++, weak to strong reactivities, respectively, based on multiple experiments under various conditions.