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. 2013 Feb;51(2):487–495. doi: 10.1128/JCM.02285-12

Table 1.

Simplified matrix for the identification of the optimal antibody combinationa

Labeling antibody Reactivity for capture antibody and antigen
1031
1061
1101
1321
1401
1451
1631
1711
1771
1841
1881
Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923 Recombinant LukF Culture supernatant LukF-P83 Culture supernatant ATCC 25923
1031 0 (+) 0 0 0 0 0 + 0 0 (+) 0 (+) + 0 (+) ++ (+) 0 0 0 0 0 (+) + ++ (+) + ++ (+) 0 (+) (+)
1061 0 (+) 0 0 0 0 0 + 0 (+) ++ 0 0 + 0 0 (+) 0 0 0 0 0 + 0 0 + (+) 0 + (+) 0 (+) (+)
1101 0 + 0 0 (+) 0 0 (+) (+) 0 ++ 0 0 (+) +++ + ++ 0 0 0 0 0 + 0 + ++ 0 ++ ++ ++ 0 (+) 0
1321 0 + 0 0 (+) 0 (+) ++ 0 0 (+) 0 0 (+) 0 ++ +++ 0 0 0 0 0 0 0 ++ +++ (+) +++ +++ 0 0 + 0
1401 + ++ 0 0 + 0 0 + ++ 0 + 0 0 (+) ++++ ++ +++ + 0 (+) 0 0 (+) 0 ++ +++ + +++ +++ +++ 0 ++ 0
1451 (+) ++ 0 0 0 0 ++ +++ (+) +++ +++ 0 + ++ ++ 0 ++ 0 0 (+) 0 ++ +++ 0 (+) ++ (+) + +++ (+) 0 + 0
1631 0 0 0 0 0 0 0 0 0 0 (+) (+) 0 0 0 0 (+) 0 0 0 0 0 0 0 0 (+) (+) 0 (+) (+) 0 (+) (+)
1711 0 ++ 0 0 + 0 (+) ++ 0 0 + 0 0 + 0 +++ +++ (+) 0 0 0 0 (+) 0 +++ +++ (+) +++ ++++ (+) + ++ ++
1771 + ++ 0 0 0 0 ++ +++ (+) +++ ++++ 0 + +++ ++ (+) ++ (+) 0 (+) 0 ++ +++ 0 + ++ (+) + +++ (+) 0 ++ (+)
1841 + +++ 0 0 0 0 +++ ++++ ++ +++ ++++ 0 ++ +++ +++ 0 ++ 0 0 (+) 0 +++ ++++ 0 + ++ 0 + +++ + 0 ++ 0
1881 0 (+) (+) 0 0 0 0 (+) 0 0 (+) 0 0 (+) 0 0 (+) (+) 0 (+) (+) 0 (+) (+) 0 (+) (+) 0 (+) (+) 0 (+) (+)
a

0, No reactivity; (+) to +++, weak to strong reactivities, respectively, based on multiple experiments under various conditions.