Table 3.
LoDs of two-step pan-HPeV and EV assays with or without MS2 internal control
| Assay and viral stock dilution (no. of CCID50s/ml) | Without MS2 ICc |
With MS2 IC |
|||
|---|---|---|---|---|---|
| No. detected/total no. (%) | Mean CT ± SD | No. detected/total no. (%) | Mean CT ± SD | Mean MS2 IC CT | |
| Pan-HPeV assaya | |||||
| 102.6 | 6/6 (100) | 32.9 ± 1.4 | 6/6 (100) | 31.3 ± 0.8 | 31.3 |
| 101.6 | 6/6 (100) | 36.1 ± 1.2 | 6/6 (100) | 36.6 ± 1.5 | 31.4 |
| 100.6 | 6/6 (100) | 39.1 ± 0.8 | 1/6 (17) | NAd | 31.7 |
| 100.06 | 0/6 (0) | NA | 0/6 (0) | NA | 31.6 |
| EV assayb | |||||
| 101.2 | 6/6 (100) | 29.9 ± 0.2 | 6/6 (100) | 30.7 ± 0.3 | 31.6 |
| 100.2 | 6/6 (100) | 32.9 ± 0.4 | 6/6 (100) | 33.6 ± 0.4 | 31.8 |
| 100.02 | 6/6 (100) | 36.3 ± 0.9 | 6/6 (100) | 37.3 ± 0.6 | 32.0 |
| 100.002 | 0/6 (0) | NA | 0/6 (0) | NA | 31.9 |
a
The HPeV3 strain was obtained from ZeptoMetrix with an initial viral titer of 1 × 108.6 CCID50s/ml. The virus culture was serially diluted with universal viral transport medium and tested at appropriate viral titers.
b
The echovirus 9 strain was obtained from ATCC with an initial viral titer of 1 × 107.2 CCID50s/ml. The virus culture was serially diluted with universal viral transport medium and tested at appropriate viral titers.
c
IC, internal control.
d
NA, not applicable.