Table 4.
LoDs of two-step pan-HPeV and -EV assays
| Viral strain | LoD in no. of CCID50s/ml (dilution)a | Initial titer (no. of CCID50s/ml) |
|---|---|---|
| HPeV types | ||
| HPeV1 | 100.001 (10−7) | 105 |
| HPeV2 | 100.005 (10−7) | 105.5 |
| HPeV4 | 100.01 (10−7) | 106 |
| HPeV5 | 100.005 (10−8) | 106.5 |
| HPeV6 | 100.1 (10−6) | 106 |
| EV types | ||
| Coxsackievirus A9 | 100.002 (10−7) | 105.2 |
| Coxsackievirus B1 | 100.04 (10−7) | 107.4 |
| Coxsackievirus B2 | 101 (10−8) | 109 |
| Coxsackievirus B3 | 100.005 (10−7) | 106.5 |
| Coxsackievirus B4 | 100.05 (10−8) | 107.5 |
| Coxsackievirus B5 | 100.5 (10−8) | 108.5 |
| Echovirus 3 | 100.0007 (10−8) | 105.7 |
| Echovirus 4 | 100.002 (10−7) | 105.2 |
| Echovirus 5 | 100.02 (10−8) | 107.2 |
| Echovirus 6 | 102.2 (10−4) | 106.2 |
| Echovirus 7 | 100.002 (10−8) | 106.2 |
| Echovirus 11 | 100.01 (10−8) | 107 |
a
Both HPeV and EV types were serially diluted in 10-fold dilutions from the initial titer and tested with pan-HPeV and EV assays in the two-step format with the AGP enzyme mix. Data in parentheses represent the nth dilution.