Fig 2.

Molecular phylogenetic analysis of the complete fusion (F) gene sequences of NDV-DR499-31/08, NDV-DR867-2/08, NDV-DR28138-4/86, and NDV-Mex452/47 among 25 other taxa available in GenBank. The evolutionary history was inferred by using the maximum likelihood method based on the general time-reversible model (40). The tree with the highest log likelihood (−12,053.7125) is shown. A discrete gamma distribution was used to model evolutionary rate differences among sites (5 categories [+G, parameter = 0.5839]). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 43.4250% sites). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 29 nucleotide sequences. Codon positions included were the first, second, third, and noncoding positions. All positions containing gaps and missing data were eliminated. There were a total of 1,652 positions in the final data set. Evolutionary analyses were conducted with MEGA5 (37).