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Nucleotides 982–994 in vRNA 1 interact with nucleotides 2075–2087 in vRNA 2. ODNs were used to map region 1939–2208 of vRNA 2 (A) and region 781–1170 of vRNA 1 (B). (C) Proposed interactions between vRNAs 1 and 2. (D) Compensatory mutations introduced in vRNAs 1 and 2 to test interaction 1. (E) Effects of mutations in vRNAs 1 or/and 2 on the intermolecular interaction.
