Figure 2.

Retention kinetics of GFP-RECQL5 at laser-induced DSBs in the absence of BLM, WRN and RECQL4. (A) Western blots showing stable knockdown of RECQL4, BLM and WRN by lentiviral shRNA in HeLa cells. (B) Retention of GFP-RECQL5 in the absence of BLM, WRN and RECQL4. Stable knockdown cells of RECQL4, BLM and WRN were transiently transfected with GFP-RECQL5 and micro-irradiated at 7% laser intensity, and retention was performed over several hours using the specially designed environmental chamber. (C) Plot comparing the retention kinetics of GFP-RECQL5. The retention was shown up to 8 h (while it retains even longer). The data points represent the mean of three independent experiments (8–10 cells analysed per experiment).