Figure 9.

Double depletion of RECQL5 and WRN in WI38 fibroblasts. (A) Transient depletion of WRN (using siRNA–WRN) was performed in stable RECQL5 knockdown cells of WI38 fibroblasts. The depletion efficiency was ∼90%. (B) Growth assays indicate poor survival of double-depleted WI38 fibroblasts compared with the single depletions. The number of cells were counted and plotted against number of days. The plot represents the mean of three independent experiments. (C) Bright-field images of single- and double-depleted WI38 fibroblasts are shown. (D) DNA fiber assays were performed with the previously generated single- and double-depleted cells of RECQL5 and WRN. (E) Enlarged images of DNA fibers of single- and double-depleted cells co-stained with DNA. BrdU-labeled fibers were stained in green and DNA in red.