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. 2012 Dec 5;41(2):1273–1283. doi: 10.1093/nar/gks1228

Figure 4.

Figure 4.

Knockdown of miR-192 blunted the increase of urine output in condition of high salt intake in mice. (A) LNA-modified anti-miR-192 decreased miR-192 expression in kidney cortex in mice. Anti-miR-192 (anti-192) or anti-scrambled control (anti-neg) oligonucleotides were delivered to mice by intraperitoneal injection (10 mg/kg body weight). Forty-eight hours after injection, miR-192 expression was suppressed to ∼30% of control levels. n = 6, *P < 0.05 versus anti-neg. (B) Knockdown of miR-192 significantly attenuated the increase of urine output when mice were switched from a 0.4% NaCl diet to a 4% NaCl diet. All animals were fed with a 0.4% NaCl diet after receipt. After 4 days of acclimatization, 24-h urine collection was started and urine output on the 0.4% NaCl diet was measured for 5 days to ensure a stable baseline. Mice were then divided into four groups based on oligonucleotide and dietary treatment: (i) remaining on the 0.4% NaCl diet and receiving scrambled anti-miR (anti-neg) (n = 6); (ii) remaining on the 0.4% NaCl diet and receiving anti-miR-192 (anti-192) (n = 6); (iii) switched to a 4% NaCl diet and receiving anti-neg (n = 8) and (iv) switched to a 4% NaCl diet and receiving anti-192 (n = 8). *P < 0.05 versus the ‘anti-neg, 4% NaCl’ group.