. 2012 Dec 2;41(2):1058–1070. doi: 10.1093/nar/gks1073

Table 3.

Effect of Zn1 on equilibrium dissociation constants from fluorescence anisotropy titrations (µM)

Enzyme + substrate	−nucleotide	+ADPNP	+ADP
rgyr_hel + ssDNA^a	0.2 ± 0.01 (n = 1.4)^b	0.46 ± 0.03 (n = 1.4)^b	0.28 ± 0.01 (n = 1.6)^b
rgyr_hel + dsDNA^a	3.9 ± 0.6	0.19 ± 0.03	3.7 ± 0.5
rgyr_hel_Zn1 + ssDNA	1.3 ± 0.4	1.3 ± 0.3	3.4 ± 0.4
rgyr_hel_Zn1 + dsDNA	not detected	3.1 ± 0.2	10 ± 2

^aFrom previous study (21).

^bBinding curves for ssDNA were analyzed using the Hill equation with Hill coefficient n.