Figure 2.

In vitro SAM-binding of Rrp8. Recombinant 6× HIS–Rrp8, 6× HIS–Rrp8–G209R and 6× HIS–Rrp8–G209A were expressed in E. coli. The purified proteins (25–30 μg) were then incubated with [³H-methyl] SAM, and the reaction mixture was exposed to UV irradiation. The products were re-solved on a 12% SDS–PAGE gel and stained with Coomassie blue. After destaining, the gel was prepared for fluorography.