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. 2012 Nov 22;41(2):1151–1163. doi: 10.1093/nar/gks1102

Figure 3.

Figure 3.

Complementation assay of tc-GAR1 with different GAR1 constructs. After transforming the plasmids expressing the whole Gar1 protein (GAR1), a Gar1 protein without the N-terminal GAR domain (ΔGAR1), a Gar1 protein without the C-terminal GAR domain (GAR1Δ) and a protein without both GAR domains (ΔGAR1Δ), ten-fold serial dilutions of the single mutants (rrp8-_C, tc-GAR1) and the double mutant (rrp8-_C tc-GAR1) were spotted onto -ura plates with or without tetracycline. Plates were incubated at 30°C.