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. 2012 Nov 22;41(2):1151–1163. doi: 10.1093/nar/gks1102

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/3.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 9. — Polysome profiles and growth analysis of the rRNA mutant strain. (A) Polysome profile analysis was performed to detect the translational status of the rRNA mutant A645U. Mutated rRNA was expressed from the plasmid pPK618 in a strain where genomic rDNA was deleted. Wild-type rRNA was expressed from pAV164. (B) Thermosensitivity of strains carrying these plasmids was analysed on solid YEPD plates incubated at different temperatures. (C) Paromomycin sensitivity tests were performed as previously described.