Figure 5.

Spatial and temporal analysis of DNA cleavage by LlaBIII and LlaGI. (A) Rates of DNA cleavage using plasmid (SC) or each linear DNA were determined using the combinations of LlaGI and LlaBIII indicated (‘Materials and Methods’ section). Points are averages with error bars as standard deviation for at least two repeat reactions. Points are joined with straight lines to guide the eye. (B) For the linear DNA substrates, gel images at each time point were quantified using ImageQuant TL. DNA marker lanes were used to calibrate and correct the pixel positions to DNA lengths. The y-axis intensity values were calculated as a percentage relative to the uncut FLL bands.