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Schematic diagram showing the strategy for Tol2 excision analysis and large genomic deletion screening using Et(gata2a:EGFP)pku684 as an example. The Tol2 transposon was considered to be excised from the original insertion site in embryos with negative or altered EGFP patterns. Asterik, PCR band containing relatively large genomic deletions; WT, wild-type. Et(gata2a:EGFP)pku684 is simplified to pku684.
