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. 2012 Nov 7;41(2):e36. doi: 10.1093/nar/gks1035

Figure 2.

Figure 2.

Analysis of footprints and genomic deletions induced by Tol2 excision in zebrafish founder embryos. (a) Representative footprints detected at the lmo2 locus after Tol2 transposon excision. lFT1: precise excision; lFT2-5: imprecise excision. The arrow indicates the direction of transcription of lmo2 gene. (b) Representative footprints detected at the nav3 locus after Tol2 transposon excision. nFT1: precise excision; nFT2-5: imprecise excision. The arrow indicates the direction of transcription of nav3 gene. (c) Schematic diagram showing the 1093-bp and 1253-bp genomic deletions at the lmo2 locus induced by Tol2 excision. Inverted letters indicate that these elements (miniP and EGFP) were inserted reversely into the endogenous gene. Dashed lines indicate deletions. (d) Schematic diagram showing the 1340-bp genomic deletion at the nav3 locus induced by Tol2 excision. Inverted letters indicate that these elements (Tol2 5′ and Tol2 3′) were inserted reversely into the endogenous gene. Dashed lines indicate deletions. The 8-bp genomic duplication is shown in uppercase. WT, wild-type. Et(gata2a:EGFP)pku684 is simplified to pku684; Et(gata2a:EGFP)pku760 is simplified to pku760.