Figure 5.

The effect of ATM kinase and TGFβ receptor signaling on pSmad2 foci formation after IR. (A) 82-6 cells were pretreated with DMSO, SD208 or KU55933 for 1 h prior to 1 Gy of γ-rays. Shown are the foci co-localization of γH2AX (green) and pSmad2 (red) 6 h after radiation. (B) 82-6 cells were pretreated with SD208 prior to 1 Gy of O ions. Other details as in (A). (C) Co-localization of γH2AX (green) and pSmad2 (red) at various times after 1 Gy of O ions in normal fibroblasts (82-6) and in (D) AT cells. Slides were irradiated vertical to the beam axis for these experiments.