Figure 2.

Interactions between Aly, THO and UAP56 are interdependent. (A) Western analyses of immunodepleted HeLa nuclear extracts with indicated antibodies. (B) RNased Mock (left panel) or ΔUAP56 nuclear extracts (right panel) were used for IPs with indicated antibodies followed by western analyses using Thoc2, UAP56 and Aly antibodies. (C and D) Same as (B), except that ΔAly and ΔTHO nuclear extract was used instead of ΔUAP56 nuclear extract, respectively. (E) Purified recombinant His-Aly or His-AlyΔC proteins were added to ΔAly extract and IPs were performed with indicated antibodies followed by western analyses.