Figure 5.

Immunolocalization of BRCA2 and RAD51 in T. brucei. Representative images are shown of BRCA2 and RAD51 localization in individual PCF TREU927 T. brucei grown in the absence of phleomycin (no BLE) or after exposure to phleomycin (2 μg ml⁻¹) for 5 h (BLE). BRCA2 is an endogenously expressed Myc-tagged variant (BRCA2myc) and was detected with anti-Myc Alexa Fluor 488-conjugated antiserum (1:7000 dilution). RAD51 was detected with polyclonal anti-RAD51 antiserum (1:1000 dilution) and secondary hybridization with Alexa Fluor 594 conjugated anti-rabbit antiserum (1:7000 dilution). Each cell is shown after staining with DAPI and as a merge of the BRCA2myc and RAD51 images (Merge). White arrows indicate co-localization between BRCA2myc and RAD51 signals.