Figure 6.

Comparative recombination activity of Vika in mammalian cells. (A) Scheme of the GFP-based recombination reporter assay. The non-recombined reporter plasmid constitutively expresses the neomycin resistance gene (NeoR). On recombination, NeoR is removed, resulting in expression of the GFP driven from the cytomegalovirus promoter (CMV prom). Triangles depict lox sites. (B) Recombination assay of Cre, VCre and Vika in HeLa cells. Images show cells stained with Hoechst 33342 (blue) and imaged for EGFP expression (green), 24 h after co-transfection with indicated recombinase expression and reporter plasmids. (C) Quantification of EGFP-positive cells for indicated recombinase expression and reporter plasmids from B, n = 3. Error bars indicate standard deviation of the mean value.