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. 2012 Nov 22;41(2):711–726. doi: 10.1093/nar/gks1136

Figure 5.

Figure 5.

Expression profile of Vglut2, GAD65 and BLBP during neuronal differentiation. Expression analysis in two inducible non-tagged clones derived from parental ES cell line EB3 (left panels) and in two knock-down clones derived from parental ES cell line E14 (right panels) was evaluated by q-PCR: Vglut2 is a glutamatergic neuron marker (A), GAD65 is a GABAergic neuron marker (B), BLBP is a radial glia marker (C). The red bar represents the control, the green bar represents the expression profile of each transcript in the overexpressing and in the knock-down clones. For each expression graph, we reported on the x-axis the days of the differentiation protocol and on the y-axis the relative expression of the transcript expressed as 2-dCT. For each expression profile, the llr was calculated, and the value was reported on the top of each graph. The llr represents the statistical significance value of the difference in the expression profiles of each transgene in the inducible and in the knock-down condition compared with each control. The statistical significance is indicated by asterisk (llr > 0).