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. 2012 Nov 22;41(2):711–726. doi: 10.1093/nar/gks1136

Figure 6.

Figure 6.

Expression of E13 in developing mouse brain. (A) Expression of E13 during mouse brain development evaluated by RNA ISH at embryonic Day 12.0 (E12.0). High levels of E13 are detected in the dorsal pallium from the ventricular to the pial surface, along the entire rostro-caudal (R-C) axis. In the ventral pallium, E13 expression is restricted to the ventricular zone of the ganglionic eminences (dashed lines). Scale bar: 500 µm. (B) High magnification of E12.0 embryonic brain at the rostro-caudal level indicated by the asterisk in (A). A graded expression of E13 is clearly visible in all the cortical primordium. Double ISH/IHC shows that although at low levels of expression. E13 levels gradually increase and co-localize with the Tbr2+ intermediate progenitor cells of the subventricular zone, which are about to undergo the final neurogenic division. The maximum rate of E13 is evident in the post-mitotic neurons of the preplate that are positive to NeuN staining (arrows and arrow heads in the higher magnification and insets). Please note that in the ganglionic eminence, E13 is detected only in the ventricular zone. Scale bars: 100 µm, 50 µm in high magnifications. ctx, cortex; hp, hippocampus; vz, ventricular zone; svz, subventricular zone; ppl, preplate; LGE, lateral ganglionic eminence; MGE, medial ganglionic eminence; CGE, caudal ganglionic eminence; mz, mantle zone; Th, thalamus; Hyp, hypothalamus.