Table 2.
Proteins identified from the co-IP experiment as potential partners of E13
| UniProt ID | Gene symbol | Protein name | Mowse score | Molecular weight (Da) | Sample name* |
|---|---|---|---|---|---|
| BTF3_MOUSE | Btf3 | Basic TF 3 | 47 | 22 017 | IC |
| C1QBP_MOUSE | C1qbp | Complement component 1 Q subcomponent-binding protein | 53 | 30 994 | IC |
| CPSF6_MOUSE | Cpsf6 | Cleavage and polyadenylation specificity factor subunit | 31 | 59 116 | IC |
| CQ096_MOUSE | E130012A19Rik | Uncharacterized protein C17orf96 homologue | 103 | 38 071 | IC |
| DDX5_MOUSE | Ddx5 | Probable ATP-dependent RNA helicase DDX | 57 | 69 277 | IC |
| EED_MOUSE | Eed | Polycomb protein EED | 53 | 50 166 | IC |
| H12_MOUSE | Hist1h1c | Histone H1.2 | 76 | 21 254 | IC |
| HS90A_MOUSE | Hsp90aa1 | Heat shock protein HSP 90-alpha | 35 | 84 735 | IC |
| IGH1M_MOUSE | Ighg1 | Ig gamma-1 chain C region, membrane-bound form | 601 | 43 359 | IC |
| IGKC_MOUSE | Igkc | Ig kappa chain C region | 843 | 11 771 | IC |
| INT3_MOUSE | Ints3 | Integrator complex subunit 3 | 28 | 117 862 | IC |
| KPRA_MOUSE | Prpsap1 | Phosphoribosyl pyrophosphate synthetase-associated protein 1 | 142 | 39 407 | IC |
| KV2A7_MOUSE | LOC636468 | Ig kappa chain V-II region 26-10 | 796 | 12 265 | IC |
| ML12B_MOUSE | Myl12b | Myosin regulatory light chain 12B | 776 | 19 767 | IC |
| MTF2_MOUSE | Mtf2 | Metal-response element-binding TF 2 | 42 | 66 882 | IC |
| MYH9_MOUSE | Myh9 | Myosin-9 | 2487 | 226 232 | IC |
| NEK9_MOUSE | Nek9 | Serine/threonine-protein kinase Nek9 | 32 | 107 015 | IC |
| NKTR_MOUSE | Nktr | NK-tumour recognition protein | 32 | 163 341 | IC |
| PCNA_MOUSE | Pcna | Proliferating cell nuclear antigen | 72 | 28 766 | IC |
| RBBP4_MOUSE | Rbbp4 | Histone-binding protein RBBP4 | 38 | 47 625 | IC |
| RL23A_MOUSE | Rpl23a | 60S ribosomal protein L23a | 240 | 17 684 | IC |
| RL28_MOUSE | Rpl28 | 60S ribosomal protein L28 | 98 | 15 724 | IC |
| RS11_MOUSE | Rps11 | 40S ribosomal protein S11 | 82 | 18 419 | IC |
| RS13_MOUSE | Rps13 | 40S ribosomal protein S13 | 105 | 17 212 | IC |
| SETX_MOUSE | Setx | Probable helicase senataxin | 53 | 297 401 | IC |
| SPTA2_MOUSE | Sptan1 | Spectrin alpha chain, brain | 658 | 284 422 | IC |
| SPTB2_MOUSE | Sptbn1 | Spectrin beta chain, brain 1 | 607 | 274 052 | IC |
| SUZ12_MOUSE | Suz12 | Polycomb protein Suz12 | 78 | 82 974 | IC |
| T2EB_MOUSE | Gtf2e2 | General TF IIE subunit 2 | 42 | 33 026 | IC |
| THOC4_MOUSE | Thoc4 | THO complex subunit 4 | 84 | 26 924 | IC |
| UBIQ_MOUSE | Rps27a | Ubiquitin | 105 | 8560 | IC |
| ACTB_MOUSE | Actb | Actin, cytoplasmic 1 | 247 | 41 710 | CTL |
| HNRPF_MOUSE | Hnrnpf | Heterogeneous nuclear ribonucleoprotein F | 170 | 45 701 | CTL |
| IGH1M_MOUSE | Ighg1 | Ig gamma-1 chain C region, membrane-bound form | 699 | 43 359 | CTL |
| IGKC_MOUSE | Igkc | Ig kappa chain C region | 306 | 11 771 | CTL |
| KV2A7_MOUSE | LOC636468 | Ig kappa chain V-II region 26-10 | 796 | 12 265 | CTL |
| MYH10_MOUSE | Myh10 | Myosin-10 | 1883 | 228 855 | CTL |
| PRPS1_MOUSE | Prps1 | Ribose-phosphate pyrophosphokinase 1 | 123 | 34 826 | CTL |
| RS5_MOUSE | Rps5 | 40S ribosomal protein S5 | 84 | 22 875 | CTL |
| IGH1M_MOUSE | Ighg1 | Ig gamma-1 chain C region, membrane-bound form | 699 | 43 359 | P-CTL |
| IGKC_MOUSE | Igkc | Ig kappa chain C region | 310 | 11 771 | P-CTL |
| KV2A7_MOUSE | LOC636468 | Ig kappa chain V-II region 26-10 | 368 | 12 265 | P-CTL |
| PPB_ECOLI | phoA | Alkaline phosphatase OS = E. coli (strain K12) | 6665 | 49 408 | P-CTL |
| IGH1M_MOUSE | Ighg1 | Ig gamma-1 chain C region, membrane-bound form | 625 | 43 359 | N-CTL |
| IGKC_MOUSE | Igkc | Ig kappa chain C region | 735 | 11 771 | N-CTL |
| KV2A7_MOUSE | LOC636468 | Ig kappa chain V-II region 26-10 | 535 | 12 265 | N-CTL |
IC = inducible cell line; CTL = control cell line; P-CTL = positive control with flag-tagged BAP protein; N-CTL = negative control without protein.
Two inducible cell samples, two control cell samples, one negative control and one positive control were processed simultaneously.
Protein hits corresponding to P < 0.05 (Mowse score >28) were considered as significant protein identifications.